Vitamin B6

 

Pyridoxal 5-phosphate (PLP), the biologically active form of vitamin B6, is an essential cofactor for multiple enzymes. The metabolically active form of vitamin B6, is an essential co-enzyme for more than 100 enzyme reactions and plays a role in macronutrient metabolism, neurotransmitter synthesis, histamine synthesis, hemoglobin synthesis and gene expression. Plasma Vitamin B6 levels are high in infants but decrease with age. Deficiency usually occurs in association with other nutrient deficiencies and intervention on healthy older people. The dependence upon exogenous sources to maintain adequate levels of Vitamin B6 in the body makes it clinically desirable to measure pyridoxal 5'-phosphate. Vitamin B6 is a water soluble compound naturally occurring in various foods, used as an additive, or available as a dietary supplement.

 

Vitamin B6 deficiency is rare but often occurs in association with low concentrations of other B-complex vitamins such as vitamin B12 and folate. Populations most commonly at risk for vitamin B6 deficiency include the elderly, inflammatory diseases, cardiovascular disease, rheumatoid arthritis, inflammatory bowel disease, and diabetes. Low vitamin B6 levels have also been linked to various conditions including cancer, PMS and cognitive function. Vitamin B6 deficiency can lead to anemia, dermatitis, glossitis, depression, confusion, and weakened immune function. A/C enzymatic vitamin B6 assay provides an efficient and cost effective method for routine and high thought-out vitamin B6 analysis, correlating well with HPLC but eliminating radiioactive , time consuming measurement on complex equipment. The assay dependent the PLP-dependent recombinant enzyme homocysteine a,g-lyase (rHCYase) which can be performed in a microtiter plate .

 

The new enzymatic vitamin B6 assay is the first homogeneous, non-radiactive B6 diagnostic applicable on chemistry auto- analyzers and should be of wide-based clinical use.

 

Applications:

Determination of vitamin B6 status
Homocysteinaemia
Inflammation
Anaemia, depression

For more info see the brochure on Vitamin B6.