B6 Kits

The A/C Enzymatic Vitamin B6 Assay reagent is a liquid stable system, which provides a fast, accurate cost effective alternative to cumbersome and expensive Radioactivity  methods. A choice of convenient instrument specific packaging options  and  easy to adapt on liquid handing robots. Accurate and reliable, the assay features excellent correlation, and  with a linear range of 10-200 nmole/L and inter and intra of <6.5 %CV and  of < 10%. The assay has no significant interference from lipemic and hemolytic substances.

96 well plate format 10 l of sample

Endpoint measurement at 675 nm (660-680nm)

Measurement Range of 15.6 250 nmol/L

Easy to adapt on liquid handing robots.

High-throughput

The A/C Enzymatic B6 Assay successfully measured vitamin B6 levels in large US population for CDC-NHANES (2003-2004).

 

 

Assay Protocol

 

Step 1:    10 l of sample
                    

                     
                    Add 150 l of Working Binding Buffer

                  Shake and Incubate at 37oC for 60 minutes
Step 2:
                    Add 100 l of Working Assay Buffer
                  Shake and Incubate at 37oC for 20 minutes
Step 3

 

                        Add 25 l of Chromogen RI and 15 l of Chromogen RII
                  Shake and Incubate at 37oC for 10 minutes
                     

                Read at between 660- 680nm wavelength.