The A/C Enzymatic
Vitamin B6 Assay reagent is a liquid stable system, which
provides a fast, accurate cost effective alternative to
cumbersome and expensive Radioactivity methods. A choice of
convenient instrument specific packaging options and easy to
adapt on liquid handing robots. Accurate and reliable, the assay
features excellent correlation, and with a linear range of
10-200 nmole/L and inter and intra of <6.5 %CV and of < 10%.
The assay has no significant interference from lipemic and
•96 –well plate format •10
µl of sample
measurement at 675 nm (660-680nm)
Range of 15.6 – 250 nmol/L
to adapt on liquid handing robots.
A/C Enzymatic B6 Assay successfully measured vitamin B6
levels in large US population for CDC-NHANES (2003-2004).
Step 1: 10 µl
Add 150 µl of
Working Binding Buffer
Incubate at 37oC for 60 minutes
Add 100 µl of
Working Assay Buffer
Incubate at 37oC for 20 minutes
25 µl of Chromogen RI and 15 µl of Chromogen RII
Incubate at 37oC for 10 minutes
at between 660- 680nm wavelength.